Performing an oxidase tests lets us see if our bacteria have cytochrome oxidase, as participant in electron transport during respiration. To check, we simply take the old unknown bacterial plate we used to the antibiotic test and a vial of oxidase. It it turns blue right away the test is positive, but if it does not turn blue or turns blue after 30 seconds then the test is negative. Our plate only turned a little blue after a few minutes indicating that our unknown bacteria is NEGATIVE for Oxidase.
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| Negative Oxidase Results |
We also added hydrogen peroxide to the plate to see if it would react and our bacteria fuzzed a lot! This means our bacteria can use aerobic respiration.
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