Today, we retrieved our thioglycolate broth tube from the incubator to see the oxygen requirement for our unknown sample. The result is as follows:
As you can see from the image above, our unknown bacteria thrived towards the surface of the broth and did not grow at all at the bottom. There are trace amounts of bacteria towards the center, meaning our bacteria is facultative anaerobic. We were unsure whether the bacteria in the middle is there because it grew there or whether we weren't careful and accidentally shook it when handling it. If that is the case, then our bacteria would be aerobic.
When we retrieved our petri dish from the GasPak, we discovered that our bacteria did not grow in the absence of oxygen. Therefore, our bacteria is aerobic/micro-aerophilic. This means that our bacteria needs oxygen to grow!
Before we left lab, we inoculated three different petri plates: one was a starch agar plate, which tests whether the bacteria produces amylase to hydrolyze amylopectin and amylose into maltose, glucose, and dextrans. Another petri dish had skim milk and it discriminates bacteria that produce protease to hydrolyze the casein into clear amino acids. The last is the blue agar plate, which tests whether the bacteria produces lipase to break down lipids.
We also inoculated a solid motility medium to determine whether our bacteria has to means to move through the medium.
...the results will be determined next lab...
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