Today, we got to find out whether our bacteria (environmental were aerobic or anaerobic. We performed two tests to determine if the bacteria needs oxygen and to what extent: one test we used was with thioglycollate broth and the other was with the GasPak.
With the first test using the thioglycollate broth, we used the ascetic to add our unknown bacteria to the liquid broth. We then placed the inoculated broth tube in the incubator (set at 30 degrees). There, it will grow so that next lab we can determine whether our unknown sample is aerobic or anaerobic. The results can appear as follows:
1. Obligate Aerobe
2. Facultative Anaerobe
3. Aerotolerant Anaerobe
4. Obligate Anaerobe
For the second test, we used the GasPak (shown below)
To use the GasPak, first we used the aseptic technique to transfer bacteria onto a new Petri dish. Then we placed the dishes into the air-tight chamber so that no oxygen could be present. Before we placed the lid on, we inserted a methyl-blue pad inside that would act as an indicator, turning from blue to colorless in the absence of oxygen.
...And now we wait until next time to see the results...
This test is based on the fact that a strong oxidizing agent, under acidic conditions, can fully oxidize almost any organic compound to carbon dioxide, anaerobic incubator and professional X-Ray viewer supplier in china.
ReplyDelete