These were the determining characteristics when using the flow chart:
- Gram positive--narrowing it down to Corynebacterium spp., Bacillus spp., and Lactobacillus spp.
- Spore-forming--narrowing it down to Bacillus spp.
- Positive mannitol test--narrowing it down to the species B. megaterium and B. subtilis
- Positive Voges-Proskauer (VP) test-- Bacillus subtilis!!!!
Now that we know what our bacteria is, we decided to do a little research on Bacillus subtilis. Here are some interesting facts that we found:
- naturally-occuring
- widespread
- used for its natural fungicidal properties to prevent crop disease
- Used in commercial Japanese and Korean food production
- Can convert explosives into nitrogen, carbon dioxide, and water
- Used as an immunostimulatory agent in alternative medicine
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